Multifunctional membranes for lipidic nanovesicle capture

Tangential flow filtration membrane systems are employed for the isolation and concentration of extracellular vesicles. However, interfacial interactions between surface species influence flux performance. Here we propose a strategy aimed at introducing functional ligands over to improve separation process through combined size-exclusion affinity-based mechanisms, avoiding binding contaminants other non-target molecules. Polysulfone membranes were modified by nanometric coating differently functionalized copolymers with dual purpose limiting non-specific while promoting chemoselective conjugation membrane-sensing peptide ligand (BPt) lipid nanovesicles capture. Copoly azide polymer positively affects physico-chemical properties membrane, improving performance antifouling capacity. A decrease decline ratio from 38.7 ± 3.9% 21.2 2.4% an increase protein permeate (Cp) respective feed (Cf) values 0.97 was measured after c-(DMA-N3-BP-MAPS) highlighting its capability reduce adsorption. In addition, BPt-functionalized displayed high capturing efficiency towards synthetic liposomes which, notably, can be promptly released upon mild treatment divalent cation solution. Overall, our work integrates conventional TFF principles isolation, broadening perspective applications.